Radioiodinated Tyrosyl-Ouabain and Measurement of a Circulating Ouabain-like Compound
نویسندگان
چکیده
منابع مشابه
Radioiodinated tyrosyl-ouabain and measurement of a circulating ouabain-like compound.
BACKGROUND Assays for endogenous ouabain, a cardiac glycoside believed to be involved in blood pressure and volume regulation, are characterized by laboratory-specific plasma values that are measured by different assays. Because of this variability, our study focused on the development of a new (125)I-labeled ouabain derivative for RIA of high sensitivity. METHODS We generated rabbit antisera...
متن کاملRadioimmunoassays, ouabain-like material, and ouabain.
Radioimmunoassays, Ouabain-Like Material, and Ouabain To the Editor: We read with interest the article by Bauer et al1 on the changes in the plasma concentration of a ouabain-like compound associated with vigorous exercise in both humans and dogs. The authors comment on the result that is most striking to us, namely the remarkably high concentrations of the immunoassayable substance achieved wi...
متن کاملEndogenous ouabain is not ouabain.
T he concept of a circulating digitalis-like inhibitor of the sodium pump, Na + , K +-ATPase, evolved from studies performed in the 1960s. De Wardener et al 1 addressed the question of whether a small increase in the glomerular filtration rate together with changes in the concentration of the more recently discovered hormone aldosterone could explain the natriuresis that generally followed salt...
متن کاملIdentification and characterization of a ouabain-like compound from human plasma.
The plasma membrane sodium-potassium pumps that regulate intracellular sodium in most animal cells have specific, high-affinity receptors for the digitalis glycosides and their aglycones. This has fostered speculation that there is an endogenous ligand. We have purified and structurally identified by mass spectroscopy an endogenous substance from human plasma that binds with high affinity to th...
متن کاملOuabain-like activity in human cerebrospinal fluid.
Human cerebrospinal fluid has been found to mimic the effect of ouabain on net Na+ efflux and 86Rb+ influx across erythrocyte membranes and on the in vitro activity of a purified Na+/K+-ATPase (ATP phosphohydrolase, EC 3.6.1.3) derived from canine kidney. These results indicate the possible existence in human cerebrospinal fluid of an endogenous factor with ouabain-like activity, which might be...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2001
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/47.1.95